Coding
cas6

Part:BBa_K3776015

Designed by: Helene Heling Huang   Group: iGEM21_HK_SSC   (2021-10-01)


cas6

This Cas6 protein from Vibrio cholerae is part of the insertion of transposable elements by guide RNA–assisted targeting (INTEGRATE) system devised by Vo et al.(2020). The INTEGRATE system consists of CRISPR RNA-guided integrases for bacterial genome engineering (see BBa_K3776022). In this system, Cas6 is part of the TniQ-Cascade protein complex which is necessary for RNA-guided DNA integration. Cas6 is a RNase which catalyses pre-crRNA processing.(Klompe et al., 2019)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None